Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions

Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions
Their pure capabilities in intercellular communication render extracellular vesicles (EV) extremely engaging for drug supply purposes. Nonetheless, the loading effectivity of current strategies to include notably hydrophilic low molecular weight medicine of biomedical curiosity is basically unexplored, as is the influence these strategies might have on the intrinsic structural and organic vesicle properties.
Right here, completely different strategies are exploited to include hydrophilic non-membrane permeable compounds into stem cell-derived small EV, and to evaluate the vesicle traits after the completely different loading processes. When evaluating a number of strategies head-to-head, the loading capability will increase within the order saponin ≤ sonication < fusion < freeze-thawing ≤ osmotic shock.
Curiously, the structural and organic capabilities of small EV are depending on the utilized encapsulation course of, with the purposeful properties being altered at a higher extent. Due to this fact, the significance of together with further characterization parameters to probe alterations of the organic performance of small EV is clearly demonstrated. Right here, freeze-thawing and notably the osmotic shock have confirmed to be probably the most acceptable strategies for EV loading, as they obtain a excessive drug encapsulation and but protect the investigated structural and organic vesicle traits.

Conformationally versatile core-bearing detergents with a hydrophobic or hydrophilic pendant: Impact of pendant polarity on detergent conformation and membrane protein stability

Membrane protein buildings present atomic degree perception into important biochemical processes and facilitate protein structure-based drug design. Nonetheless, the inherent instability of those bio-macromolecules exterior lipid bilayers hampers their structural and purposeful examine. Detergent micelles can be utilized to solubilize and stabilize these membrane-inserted proteins in aqueous resolution, thereby enabling their downstream characterizations.
Membrane proteins encapsulated in detergent micelles are likely to denature and combination over time, highlighting the necessity for growth of recent amphiphiles efficient for protein solubility and stability. On this work, we current newly-designed maltoside detergents containing a pendant chain hooked up to a glycerol-decorated tris(hydroxylmethyl)methane (THM) core, designated GTMs. One set of the GTMs has a hydrophobic pendant (ethyl chain; E-GTMs), and the opposite set has a hydrophilic pendant (methoxyethoxylmethyl chain; M-GTMs) positioned within the hydrophobic-hydrophilic interfaces.
 Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions
The 2 units of GTMs displayed profoundly completely different behaviors by way of detergent self-assembly and protein stabilization efficacy. These behaviors primarily come up from the polarity distinction between two pendants (ethyl and methoxyethoxylmethyl chains) that ends in a big variation in detergent conformation between these units of GTMs in aqueous media. The ensuing excessive hydrophobic density within the detergent micelle inside is probably going accountable for enhanced efficacy of the M-GTMs for protein stabilization in comparison with the E-GTMs and a gold customary detergent DDM.
A consultant GTM, M-GTM-O12, was more practical for protein stability than some not too long ago developed detergents together with LMNG. That is the primary case examine investigating the impact of pendant polarity on detergent geometry that correlates with detergent efficacy for protein stabilization.

An azine-based polymer derived hierarchically porous N-doped carbon for hydrophilic dyes elimination

On this examine, a novel hierarchically porous N-doped carbon (HPNC) materials was efficiently ready by soft-templating technique. The industrial triblock copolymer of Pluronic F127 and a polyazine derived from hydrazine hydrate & glyoxal have been used as comfortable template and precursor, respectively. The obtained supplies have been totally characterised and examined as a sorbent for the elimination of hydrophilic dyes of Methylene blue (MB), Fundamental Fuchsin (BF), Eosin Y (EY) and Rhodamine B (RB) from their aqueous effluents.
Based on the characterization outcomes, the synthesized materials of HPNC-1000 offered thick fibrous morphology with micron dimension in diameter, hierarchically porous construction with floor space of 1853 m2/g, pore quantity of 1.59 cm3/g and nitrogen content material of 4.5 wt%. Adsorption-desorption investigation reveals that synergistic impact of hydrophobic interplay and hydrogen-bonding formation of the dye molecules with the sorbent was most pronounced within the adsorptions. The utmost adsorption capacities for MB, BF, EY and RB reached 0.83, 0.92, 1.23 and 1.83 mmol g-1, respectively. The adsorption processes effectively fitted by the pseudo first-order kinetic mannequin and the Liu’s isotherm. The sorbent will be regenerated by above 90% of the preliminary adsorption effectivity after six regeneration cycles.

Evaluation of Aqueous Extraction Strategies on Extractable Natural Matter and Hydrophobic/Hydrophilic Fractions of Virgin Forest Soils

The evaluation of water-extractable natural matter utilizing an autoclave can present helpful data on bodily, chemical, and organic adjustments throughout the soil. The current examine used virgin forest soils from Chini Forest Reserve, Langkawi Island, and Kenyir Forest Reserve (Malaysia), extracted utilizing completely different extraction strategies.
The dissolved natural carbon (DOC), complete dissolved nitrogen (TDN), complete dissolved phosphorus (TDP), and ammonium-nitrate content material have been greater within the autoclave remedies, as much as 3.0, 1.3, 1.2, and 1.Four occasions greater than by pure extraction (extracted for 24 h at room temperature). General, the best extractable DOC, TDN, TDP, ammonium and nitrate may very well be seen underneath autoclaved situations 121 °C 2×, as much as 146.74 mg C/L, 8.97 mg N/L, 0.23 mg P/L, 5.43 mg N mg/L and three.47 N mg/L, respectively. The soil extracts grew to become barely acidic with a better temperature and longer length.

COOH MagneZoom™ (Paramagnetic Beads)

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COOH MagneZoom™ (Paramagnetic Beads)

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MagneZoom™ (Paramagnetic Beads) Station

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MagneZoom™ (Paramagnetic Beads) Station

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MagneZoom™ (Paramagnetic Beads) Control

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MagneZoom™ (Paramagnetic Beads) Control

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Aldehyde MagneZoom™ (Paramagnetic Beads)

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Calmodulin MagneZoom™ (Paramagnetic Beads) Kit

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Protein A MagneZoom™ (Paramagnetic Beads) Kit

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EDC Coupling MagneZoom™ (Paramagnetic Beads) Kit

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Epoxy Coupling MagneZoom™ (Paramagnetic Beads) Kit

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Oligo (dT)25 MagneZoom™ (Paramagnetic Beads)

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Calmodulin (CAM) MagneZoom™ (Paramagnetic Beads)

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Triticum Vulgaris Lectin (WGA) - MagneZoom™ (Paramagnetic Beads) Kit

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Concanavalin A Lectin (Con A) - MagneZoom™ (Paramagnetic Beads) Kit

20163020-1 -
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Goat Anti-Rat IgG (Fc) Antibody MagneZoom™ (Paramagnetic Beads) Kit

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Multi-Lectin Fucose-Specific - MagneZoom™ (Paramagnetic Beads) Kit

22110028-1 -
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Multi-Lectin GlcNac-Specific - MagneZoom™ (Paramagnetic Beads) Kit

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Goat Anti-Human IgG (Fc) Antibody MagneZoom™ (Paramagnetic Beads) Kit

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Multi-Lectin Galactose-Specific - MagneZoom™ (Paramagnetic Beads) Kit

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Goat Anti-Rabbit IgG (Fc) Antibody MagneZoom™ (Paramagnetic Beads) Kit

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Goat Anti-Mouse IgG (H&L) Antibody MagneZoom™ (Paramagnetic Beads) Kit

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Multi-Lectin Sialic Acid-Specific - MagneZoom™ (Paramagnetic Beads) Kit

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Goat Anti-Rabbit IgG (H&L) Antibody MagneZoom™ (Paramagnetic Beads)

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Rna Isolation Kit

11828665001 EACH
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Total RNA Isolation Kit

P-9100 50 samples
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Total RNA Isolation Kit II

FATRS--001 100 preps
EUR 369.6

Total RNA Isolation Kit II

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EVery EV RNA Isolation Kit

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BT4184 250preps
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P-9104 50 samples
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PT4191 50Preps
EUR 95.63

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EpiMag Viral RNA Isolation Kit

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Easy3-Plant RNA Isolation Kit

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Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
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Broad Range Total RNA Isolation Kit

K1341050 1 kit
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Phenol-Free Plant RNA Isolation Kit

10760056-1 50 Prep(s)
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Easy3-RNA Isolation Kit

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EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
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RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
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FastPure Universal Plant Total RNA Isolation Kit

RC411-01 50 rxns
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FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01 50 rxns
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PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 343.2

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
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miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
  • EUR 702.06
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  • EUR 454.30
  • 100 Samples
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Exosome Isolation Kit

E601E001 20 tests
EUR 2500

MicroRNA Isolation Kit

KS341025 1 kit
EUR 247

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01 50 rxns
EUR 100

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

Protoplast Isolation Kit

30210002-2 50 Prep(s)
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CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504 1
EUR 270
Description: DNAse I from bovine pancreas (cow; Bos taurus)

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 273.97

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 283.2

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

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K5011150 1 kit
EUR 237

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K5011250 1 kit
EUR 237

Serum DNA Isolation Kit

K5018100 1 kit
EUR 355

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K5011050 1 kit
EUR 237

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 462
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 385.2

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 498
Description: Lipid droplets are lipid rich organelles found in the adipose tissue of eukaryotes. They function to regulate the hydrolysis and storage of neutral lipids and serve as storage for cholesterol and acyl-glycerols. Lipid droplets have also been associated with inflammatory responses, obesity, atherosclerosis, and cancer.

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 874.8
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

miRNA ISOLATION KIT-TISSUE

IB47370 4 PREP KIT
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miRNA ISOLATION KIT-TISSUE

IB47371 100 PREP KIT
EUR 579.66

Exosome Isolation kit (for serum & plasma -Exosome-RNA-NGS)

P101NGS - Ask for price

Exo-Urine EV Isolation Kit

EXOU100A-1 2 x 5 reactions
EUR 490

FitAmp Gel DNA Isolation Kit

P-1007 100 Samples
EUR 324.48

Cell Mitochondria Isolation Kit

abx090630-50100tests 50-100 tests
EUR 309.6

Mitochondrial DNA Isolation Kit

55R-1361 50 assays
EUR 990
Description: Mitochondrial DNA Isolation Kit for use in the research laboratory

Mitochondrial DNA Isolation Kit

K280-50 each
EUR 705.6

Mitochondrial DNA Isolation Kit

K2117-50 50 assays
EUR 718.8

Mitochondrial DNA Isolation Kit

K2117-1 1 kit
EUR 858
Description: Isolates mt. DNA, no genomic DNA contamination

Cell Mitochondria Isolation Kit

ETF3601 100 assays
EUR 135

Whole Blood DNA Isolation Kit

K528-100 each
EUR 451.2

Plant/Fungi DNA Isolation Kit

10760114-1 50 Prep(s)
EUR 350.39

Yeast Mitochondria Isolation Kit

K259-50 each
EUR 502.8

FitAmp Urine DNA Isolation Kit 

P-1017
  • EUR 474.12
  • EUR 171.60
  • EUR 292.60
  • 100 Samples
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  • 100 Samples

XCF Exosomal DNA Isolation Kit

XCF200A-1 20 rxn
EUR 328

Tissue Mitochondria Isolation Kit

abx090631-50100assays 50-100 assays
EUR 309.6

Tissue Mitochondria Isolation Kit

ETF3606 100 assays
EUR 135

Lysosome Isolation Kit (20 tests)

P534 - Ask for price

Exosome Isolation kit (for urine)

P120L - Ask for price

Exosome Isolation kit (for urine)

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FastPure FFPE DNA Isolation Kit

DC105-01 50 rxns
EUR 75.2

CD19 Positive Cell Isolation Kit

78564-1 1 x 10^8 Cells
EUR 70
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

CD19 Positive Cell Isolation Kit

78564-2 1 x 10^9 Cells
EUR 630
Description: The CD19 Positive Cell Isolation Kit is designed to magnetically separate CD19-expressing-cells from a complex immune cell population. This kit is optimized for the isolation of CD19 positive cells from normal human peripheral blood mononuclear cells (PBMCs). Cells are incubated with the antibody:bead complex and placed on a magnet for quick and easy separation. When placed on the magnet, CD19-positive cells will be immobilized along the side of the tube while undesired CD19-negative cells will remain in suspension for easy removal.

Genoirse Plant DNA Isolation Kit

GR101083 50
EUR 223

ExoPure? Isolation Kit   (Cell Media)

K1237-10 each
EUR 796.8

ExoPure? Isolation Kit   (Cell Media)

K1237-2 each
EUR 470.4

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K1241-10 each
EUR 836.4

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K1241-2 each
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K1238-10 each
EUR 836.4

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K1238-2 each
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Apoptotic DNA Ladder Isolation Kit

K170-50 each
EUR 561.6

Cell Surface Protein Isolation Kit

K295-10 each
EUR 601.2

Apoptotic DNA Ladder Isolation Kit

K2045-50 50 assays
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GPC1 Capture Exosome Isolation Kit

E601E004 20 tests
EUR 2500

CD63 Capture Exosome Isolation Kit

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Blood and Serum DNA Isolation Kit

K5017100 1 kit
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Bacterial Genomic DNA Isolation Kit

K309-100 each
EUR 451.2

Breast cancer Exosome Isolation Kit

E601E002 20 tests
EUR 2500

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EUR 2500

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EZExtract? Core Histone Isolation Kit

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10-min. Single Cell Isolation Kit

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MagIso NK Cell Isolation Kit, Human

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Circulating Nuleic Acid Isolation Kit

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Detergent-Free Nuclei Isolation Kit

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Mouse Brain Microvessel Isolation Kit

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MagIso CD3 Cell Isolation Kit, Mouse

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Org Frontier? Chloroplast Isolation Kit

K468-10 each
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Nucleic fluid extraction isolation kit

K1137-100 100rxns
EUR 160
Description: Nucleic fluid extraction isolation kit

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K1137-200 200rxns
EUR 270
Description: Nucleic fluid extraction isolation kit

Nucleic fluid extraction isolation kit

K1137-50 50rxns
EUR 90
Description: Nucleic fluid extraction isolation kit

MagIso CD90.2 Cell Isolation Kit, Mouse

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ExoQuick Exosome Isolation and RNA Purification kit (for Serum and Plasma)

EQ806A-1 20 preps
EUR 493

Easy3-Total NucleicAcid Isolation Kit

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FitAmp Plasma/Serum DNA Isolation Kit 

P-1004
  • EUR 416.70
  • EUR 149.60
  • EUR 253.00
  • 100 Samples
  • 50 Samples
  • 100 Samples

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-10 each
EUR 901.2

ExoPure? Isolation Kit   (Stem Cell Media)

K1239-2 each
EUR 510

Exosome Isolation kit (for serum & plasma)

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Rapid Fungal Genomic DNA Isolation Kit

FT71415 50Preps
EUR 91.97

Plant Tissue Genomic DNA Isolation Kit

K316-100 each
EUR 451.2

MagIso CX3CR1 Cell Isolation Kit, Mouse

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OrgFrontier? Plasma Membrane Isolation Kit

K414-10 each
EUR 679.2

Mitochondria Isolation Kit (Cultured Cells)

K1138-10 10rxns
EUR 35
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-20 20rxns
EUR 55
Description: Mitochondria Isolation Kit

Mitochondria Isolation Kit (Cultured Cells)

K1138-30 30rxns
EUR 70
Description: Mitochondria Isolation Kit

Easy3-Plant Genomic DNA Isolation Kit

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Easy3-Plant Genomic DNA Isolation Kit

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MagIso CD4 T Cell Isolation Kit, Human

WHK-B001 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Human

WHK-B002 10 tests
EUR 724.8

MagIso CD4 T Cell Isolation Kit, Mouse

WHK-B003 10 tests
EUR 724.8

MagIso CD8 T Cell Isolation Kit, Mouse

WHK-B004 10 tests
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MagIso Pan B Cell Isolation Kit, Mouse

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MagIso Pan B Cell Isolation Kit, Human

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Rapid Bacteria Genomic DNA Isolation Kit

BS8225 50Preps
EUR 91.97

Human Whole Blood Monocyte Isolation Kit

K933-30 each
EUR 451.2

Mammalian Cell Genomic DNA Isolation Kit

K967-100 each
EUR 457.2

ExoQuick Exosome Isolation and RNA Purification kit (for Tissue Culture Media)

EQ806TC-1 10 preps
EUR 493

Exosome Isolation kit (forHuman Body Fluid)

P121 - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121L - Ask for price

Exosome Isolation kit (forHuman Body Fluid)

P121S - Ask for price

MagIso Naïve B Cell Isolation Kit, Human

WHK-B024 20 tests, 200 tests Ask for price

Soil DNA Isolation Mini Kit (100prep)

FASOI-001-1 100 preps
EUR 241.2

MagIso Neutrophil Cell Isolation Kit, Mouse

WHK-B015 10 tests, 100 tests Ask for price

Soil DNA Isolation Mini Kit (50prep)

FASOI-001 50 preps
EUR 189.6

Soil DNA Isolation Midi Kit (24prep)

FASOI-002 24 preps
EUR 259.2

MagIso Pan B Cell Isolation Kit, Mouse Ⅱ

WHK-B012 20 tests, 200 tests Ask for price
Comparable developments have been noticed within the humic and nonhumic substances, the place several types of soil extract remedies influenced the concentrations of the fractions. Totally different soil extraction strategies can present additional particulars, thus widening the applying of soil extracts, particularly in microbes.

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