Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions

Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions
Their pure capabilities in intercellular communication render extracellular vesicles (EV) extremely engaging for drug supply purposes. Nonetheless, the loading effectivity of current strategies to include notably hydrophilic low molecular weight medicine of biomedical curiosity is basically unexplored, as is the influence these strategies might have on the intrinsic structural and organic vesicle properties.
Right here, completely different strategies are exploited to include hydrophilic non-membrane permeable compounds into stem cell-derived small EV, and to evaluate the vesicle traits after the completely different loading processes. When evaluating a number of strategies head-to-head, the loading capability will increase within the order saponin ≤ sonication < fusion < freeze-thawing ≤ osmotic shock.
Curiously, the structural and organic capabilities of small EV are depending on the utilized encapsulation course of, with the purposeful properties being altered at a higher extent. Due to this fact, the significance of together with further characterization parameters to probe alterations of the organic performance of small EV is clearly demonstrated. Right here, freeze-thawing and notably the osmotic shock have confirmed to be probably the most acceptable strategies for EV loading, as they obtain a excessive drug encapsulation and but protect the investigated structural and organic vesicle traits.

Conformationally versatile core-bearing detergents with a hydrophobic or hydrophilic pendant: Impact of pendant polarity on detergent conformation and membrane protein stability

Membrane protein buildings present atomic degree perception into important biochemical processes and facilitate protein structure-based drug design. Nonetheless, the inherent instability of those bio-macromolecules exterior lipid bilayers hampers their structural and purposeful examine. Detergent micelles can be utilized to solubilize and stabilize these membrane-inserted proteins in aqueous resolution, thereby enabling their downstream characterizations.
Membrane proteins encapsulated in detergent micelles are likely to denature and combination over time, highlighting the necessity for growth of recent amphiphiles efficient for protein solubility and stability. On this work, we current newly-designed maltoside detergents containing a pendant chain hooked up to a glycerol-decorated tris(hydroxylmethyl)methane (THM) core, designated GTMs. One set of the GTMs has a hydrophobic pendant (ethyl chain; E-GTMs), and the opposite set has a hydrophilic pendant (methoxyethoxylmethyl chain; M-GTMs) positioned within the hydrophobic-hydrophilic interfaces.
 Encapsulation of Hydrophilic Compounds in Small Extracellular Vesicles: Loading Capacity and Impact on Vesicle Functions
The 2 units of GTMs displayed profoundly completely different behaviors by way of detergent self-assembly and protein stabilization efficacy. These behaviors primarily come up from the polarity distinction between two pendants (ethyl and methoxyethoxylmethyl chains) that ends in a big variation in detergent conformation between these units of GTMs in aqueous media. The ensuing excessive hydrophobic density within the detergent micelle inside is probably going accountable for enhanced efficacy of the M-GTMs for protein stabilization in comparison with the E-GTMs and a gold customary detergent DDM.
A consultant GTM, M-GTM-O12, was more practical for protein stability than some not too long ago developed detergents together with LMNG. That is the primary case examine investigating the impact of pendant polarity on detergent geometry that correlates with detergent efficacy for protein stabilization.

An azine-based polymer derived hierarchically porous N-doped carbon for hydrophilic dyes elimination

On this examine, a novel hierarchically porous N-doped carbon (HPNC) materials was efficiently ready by soft-templating technique. The industrial triblock copolymer of Pluronic F127 and a polyazine derived from hydrazine hydrate & glyoxal have been used as comfortable template and precursor, respectively. The obtained supplies have been totally characterised and examined as a sorbent for the elimination of hydrophilic dyes of Methylene blue (MB), Fundamental Fuchsin (BF), Eosin Y (EY) and Rhodamine B (RB) from their aqueous effluents.
Based on the characterization outcomes, the synthesized materials of HPNC-1000 offered thick fibrous morphology with micron dimension in diameter, hierarchically porous construction with floor space of 1853 m2/g, pore quantity of 1.59 cm3/g and nitrogen content material of 4.5 wt%. Adsorption-desorption investigation reveals that synergistic impact of hydrophobic interplay and hydrogen-bonding formation of the dye molecules with the sorbent was most pronounced within the adsorptions. The utmost adsorption capacities for MB, BF, EY and RB reached 0.83, 0.92, 1.23 and 1.83 mmol g-1, respectively. The adsorption processes effectively fitted by the pseudo first-order kinetic mannequin and the Liu’s isotherm. The sorbent will be regenerated by above 90% of the preliminary adsorption effectivity after six regeneration cycles.

Evaluation of Aqueous Extraction Strategies on Extractable Natural Matter and Hydrophobic/Hydrophilic Fractions of Virgin Forest Soils

The evaluation of water-extractable natural matter utilizing an autoclave can present helpful data on bodily, chemical, and organic adjustments throughout the soil. The current examine used virgin forest soils from Chini Forest Reserve, Langkawi Island, and Kenyir Forest Reserve (Malaysia), extracted utilizing completely different extraction strategies.
The dissolved natural carbon (DOC), complete dissolved nitrogen (TDN), complete dissolved phosphorus (TDP), and ammonium-nitrate content material have been greater within the autoclave remedies, as much as 3.0, 1.3, 1.2, and 1.Four occasions greater than by pure extraction (extracted for 24 h at room temperature). General, the best extractable DOC, TDN, TDP, ammonium and nitrate may very well be seen underneath autoclaved situations 121 °C 2×, as much as 146.74 mg C/L, 8.97 mg N/L, 0.23 mg P/L, 5.43 mg N mg/L and three.47 N mg/L, respectively. The soil extracts grew to become barely acidic with a better temperature and longer length.

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MagneZoom™ (Paramagnetic Beads) Control

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Calmodulin MagneZoom™ (Paramagnetic Beads) Kit

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Aldehyde MagneZoom™ (Paramagnetic Beads)

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EUR 114.65

Aldehyde MagneZoom™ (Paramagnetic Beads)

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Protein A MagneZoom™ (Paramagnetic Beads) Kit

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EDC Coupling MagneZoom™ (Paramagnetic Beads) Kit

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Ni-IMAC MagneZoom™ (Paramagnetic Beads)

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Calmodulin (CAM) MagneZoom™ (Paramagnetic Beads)

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ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9 Capture Beads)

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Rna Isolation Kit - EACH

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Total RNA Isolation Kit

P-9100 50 samples
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ExoQualiTM Human Exosome Capture and Isolation Kits (for Serum/Plasma) (CD9/CD63 Capture Beads)

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K2031010 1 kit
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Cell RNA Isolation(200) Kit

TBS6001 200 samples
EUR 358

Tissue RNA Isolation(200) Kit

TBS6003 200 samples
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ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD9 Capture Beads)

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Total RNA Isolation Kit II

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EUR 369.6

Total RNA Isolation Kit II

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EUR 259.2

EVery EV RNA Isolation Kit

EVery100B-1 20 preps
EUR 412

Blood RNA Isolation(50) Kit     

TBS6002 50 samples
EUR 198

Viral DNA/RNA Isolation Kit

AGDK-0044 Kit Ask for price
Description: The Viral DNA/RNA Isolation Kit is intended for rapid co-extraction of viral DNA and RNA from a variety of biofluid samples, such as, plasma, serum, milk and swap samples. The proprietary microspherical paramagnetic beads used in the kit have a large binding surfaces and a high affinity towards nucleic acids. Going through sample lysis/binding, washing, and elution steps, the whole process can be completed under 35 minutes, and yields highly pure nucleic acids elute.  The recovered nucleic acids can be used in a wide range of applications, such as PCR, RT-PCR, Sanger Sequencing, NGS, and gene chips.

ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD63 Capture Beads)

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Rapid Blood RNA Isolation Kit

BT4182 10Preps
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Rapid Blood RNA Isolation Kit

BT4183 50Preps
EUR 197.03

Rapid Blood RNA Isolation Kit

BT4184 250preps
EUR 608.1

Rapid Plant RNA Isolation Kit

PT4191 50Preps
EUR 95.63

Total RNA Isolation Kit (Plant)

P-9104 50 samples
EUR 416.7

Total RNA Isolation Kit (Plant)

PDC07-0100 100 rxns
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P-9102 50 samples
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EpiMag Viral RNA Isolation Kit

P-9108-050 50 samples
EUR 152.9

EpiMag Viral RNA Isolation Kit

P-9108 50 samples
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Total RNA Isolation Kit(Tissue)

PDC08-0100 100 rxns
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Easy3-Plant RNA Isolation Kit

FYG502-100P 100 Preps Ask for price

Easy3-Plant RNA Isolation Kit

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ExoQualiTM Mouse Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (mCD9/CD63 Capture Beads)

CEIK-106L-10T 10T Ask for price

Rapid Bacteria RNA Isolation Kit

BS8625 50Preps
EUR 95.63

ExoQualiTM Human Exosome Capture and Isolation Kits (for Cell Culture Media/ Urine) (CD9/CD63/CD81 Capture Beads)

CEIK-100L-10T 10T Ask for price

Broad Range Total RNA Isolation Kit

K1341050 1 kit
EUR 298

Total RNA Isolation Reagent Kit

abx460023-1096tests 10 × 96 tests Ask for price

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abx460023-596tests 5 × 96 tests Ask for price

Total RNA Isolation Reagent Kit

abx460023-96tests 96 tests
EUR 275

Total RNA Isolation Kit (Column Based)

MBS402031-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit (Column Based)

MBS402031-4Reactions 4Reactions
EUR 190

Easy3-RNA Isolation Kit

FYG501-100P 100 Preps Ask for price

Easy3-RNA Isolation Kit

FYG501-500P 500 Preps Ask for price

Phenol-Free Plant RNA Isolation Kit

10760056-1 50 Prep(s)
EUR 170.53

Column-Pure Fungi RNA Isolation Kit

D510 50 Preparations
EUR 150

RNApure Total RNA Rapid Isolation Kit

MBS355999-INQUIRE INQUIRE Ask for price

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106-050 50 samples
EUR 163.9

EpiQuik Magbeads Quick RNA Isolation Kit

P-9106 50 samples
EUR 151

Soil RNA Isolation Mini Kit (100prep)

FASRK-100 100 preps
EUR 224.4

Soil RNA Isolation Mini Kit (50prep)

FASRK-001 50 preps
EUR 180

Total RNA Isolation Reagent

PDR03-0100 100 rxns
EUR 66

EpiQuik Total RNA Isolation Fast Kit

P-9105-050 50 samples
EUR 176

EpiQuik Viral RNA Isolation Fast Kit

P-9107-050 50 samples
EUR 167.2

EpiQuik Total RNA Isolation Fast Kit

P-9105 50 samples
EUR 163

EpiQuik Viral RNA Isolation Fast Kit

P-9107 50 samples
EUR 158

Total RNA Isolation Kit (Plant) (Column Based)

MBS402034-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit (Plant) (Column Based)

MBS402034-4Reactions 4Reactions
EUR 190

Plant Total RNA Isolation Reagent Kit

abx460024-1096tests 10 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-596tests 5 × 96 tests Ask for price

Plant Total RNA Isolation Reagent Kit

abx460024-96tests 96 tests
EUR 362.5

Total RNA Isolation Kit(Tissue) (Column Based)

MBS402035-100Reactions 100Reactions
EUR 400

Total RNA Isolation Kit(Tissue) (Column Based)

MBS402035-4Reactions 4Reactions
EUR 190

FastPure Cell/Tissue Total RNA Isolation Kit

RC101 50 rxn
EUR 274.8

RNA-easy Isolation Reagent

R701-01 100 ml
EUR 201.6

RNA-easy Isolation Reagent

R701-02 200 ml
EUR 254.4

FastPure Universal Plant Total RNA Isolation Kit

RC411-01 50 rxns
EUR 100

FastPure Universal Plant Total RNA Isolation Kit

RC411-00-10rxns 10 rxns
EUR 1.09

FastPure Universal Plant Total RNA Isolation Kit

RC411-01-50rxns 50 rxns
EUR 129.71

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__050 1 kit
EUR 62

Total RNA Isolation Kit II (Tri-reagent + RNA spin column)

FATRS__100 1 kit
EUR 112

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01 50 rxns
EUR 82.6

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-00-10rxns 10 rxns
EUR 1.09

FastPure Cell/Tissue Total RNA Isolation Kit V2

RC112-01-50rxns 50 rxns
EUR 129.44

Plant Total RNA Isolation Reagent

PDR04-0100 100 rxns
EUR 110

Total RNA Isolation Kit (Blood/Cultured Cell/Fungus)

PDC04-0100 100 rxns
EUR 220

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS004 each
EUR 3

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS050 each
EUR 83.4

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS100 each
EUR 153

rSYNC™ RNA Isolation Kit - Individual Packed Columns

RS300 each
EUR 408

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__001 1 kit
EUR 134.5

miRNA Isolation Kit (Tri-reagent + RNA spin column)

FAMIK__002 1 kit
EUR 73

Ultrapure Total RNA Rapid Isolation Kit (whole blood)

MBS356003-50Tests 50Tests
EUR 315

Total RNA Isolation Reagent (Reagent Based)

MBS402052-100mL 100mL
EUR 235

Total RNA Isolation Reagent (Reagent Based)

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EUR 165

EASYspin RNA Isolation Kit (Ultramicro-volume, with DNase I)

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EUR 455

EASYspin RNA Isolation Kit (Ultramicro-volume, with DNase I)

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PureXtract RNAsol RNA Isolation Solution, LS

R6103-120 2X60ml
EUR 343.2

EASYspin RNA Isolation Kit (For yeast)(without Phenol & Chloroform)

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EUR 395

EASYspin RNA Isolation Kit (For yeast)(without Phenol & Chloroform)

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EUR 1755

miRNA Isolation Kit

FYG304-100P 100 Preps Ask for price

miRNA Isolation Kit

FAMIK--001 Rxns: 100
EUR 398.4
Description: Contents of the kit:
  • Rxns:100Lysis
  • Buffer-10 ml
  • Acid-Phenol-10 ml
  • RNA Column and Collection Tube-100 sets
  • Wash Buffer-10 ml
  • Release Buffer-0.5 ml

miRNA Isolation Kit

FAMIK--002 Rxns: 50
EUR 267.6

Exosome Isolation Kit

E601E001 20 tests
EUR 2500

Exosome Isolation Kit

EIK01 2T
EUR 800
Description: Chromatography Reagents

Exosome Isolation Kit

MBS8579443-20Tests 20Tests
EUR 2625

Exosome Isolation Kit

MBS8579443-5x20Tests 5x20Tests
EUR 12240

MicroRNA Isolation Kit

KS341025 1 kit
EUR 284

Endosome Isolation Kit

P528 - Ask for price

Microsome Isolation Kit

K249-50 each
EUR 633.6

Protoplast Isolation Kit

30210002-1 20 Prep(s)
EUR 199.16

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30210002-2 50 Prep(s)
EUR 438.32

Chloroplast Isolation Kit

30210000-1 20 Prep(s)
EUR 273.97

Genomic DNA Isolation Kit (Paraffin-embedded tissue)

PDC12-0100 100 rxns
EUR 132

Mitochondria Isolation Kit

AR0156 1 kit
EUR 127.2

Preadipocyte Isolation Kit

K583-5 each
EUR 639.6

Mitochondria Isolation Kit

BC156-50T 50T
EUR 150

EASYspin RNA Isolation Kit (For Bacteria)(with DNA removation columns)

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EUR 475

EASYspin RNA Isolation Kit (For Bacteria)(with DNA removation columns)

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EUR 2135

FitAmp Paraffin Tissue Section DNA Isolation Kit 

P-1009
  • Ask for price
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  • Ask for price
  • 100 Samples
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  • 100 Samples

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD004 each
EUR 5

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD050 each
EUR 103.4

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD100 each
EUR 193

rSYNC™ RNA Isolation Kit w/DNase Set - Individual Packed Columns

RSD300 each
EUR 518

Plant Total RNA Isolation Reagent (Reagent Based)

MBS402053-100mL 100mL
EUR 245

Plant Total RNA Isolation Reagent (Reagent Based)

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EUR 165

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01 50 rxns
EUR 100

FastPure Plant Total RNA Isolation Kit (Polysaccharides&Polyphenolics –rich)

RC401-01-50rxns 50 rxns
EUR 129.44

Urine DNA Isolation Kit

K5011150 1 kit
EUR 261

Stool DNA Isolation Kit

K5011250 1 kit
EUR 261

Serum DNA Isolation Kit

K5018100 1 kit
EUR 391

EASYspin RNA Isolation Kit (For Tissues & Cells)(Without Phenol & Chloroform)

MBS355950-50Tests 50Tests
EUR 435

EASYspin RNA Isolation Kit (For Tissues & Cells)(Without Phenol & Chloroform)

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EUR 1935

PLANTpure Total RNA Rapid Isolation Kit (plants tissues or cells)

MBS356000-INQUIRE INQUIRE Ask for price

Saliva DNA Isolation Kit

AG0226 Kit Ask for price
Description: Saliva DNA Isolation Kit provides a fast and simple spin column procedure for isolating high quality DNA from saliva samples collected and preserved using AcceGen's Saliva DNA Collection and Preservation Devices, as well as fresh saliva samples.Saliva DNA purified using AcceGen's kit is of the excellent quality, and is compatible with a number of downstream research applications including PCR, qPCR, Southern Blot analysis, sequencing and microarray analysis.

Saliva DNA Isolation Kit

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EUR 261

FastPure Plant Total RNA Isolation Kit (Polysaccharides/Polyphenolics-Rich)

RC401 50 rxn
EUR 283.2

Genomic DNA Isolation Kit

55R-1362 50 assays
EUR 273
Description: Genomic DNA Isolation Kit for use in the research laboratory

Genomic DNA Isolation kit

MBK0069 50 TEST
EUR 366.24

Genomic DNA Isolation Kit

K2118-50 50 assays
EUR 389
Description: Isolates highly pure Genomic DNA within 90 min.

Genomic DNA Isolation Kit

GWB-AXR187 50 assays Ask for price

Genomic DNA Isolation Kit

K281-50 each
EUR 326.4

Genomic DNA Isolation Kit

abx460025-1096tests 10 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-596tests 5 × 96 tests Ask for price

Genomic DNA Isolation Kit

abx460025-96tests 96 tests
EUR 437.5

EASYspin RNA Isolation Kit (For Fibrous Tissues)(without Phenol & Chloroform)

MBS355952-50Tests 50Tests
EUR 485

EASYspin RNA Isolation Kit (For Fibrous Tissues)(without Phenol & Chloroform)

MBS355952-5x50Tests 5x50Tests
EUR 2190

Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

208601 8 pcs/cs
EUR 131.12
Description: Magnetic Pathogens Nucleic Acid (DNA/RNA)Isolation Kit, 50 reactions/pk

Yeast Nuclei Isolation Kit

K289-50 each
EUR 470.4

Camelid PBMC isolation Kit

MBS541616-1Kit 1Kit
EUR 585

Camelid PBMC isolation Kit

MBS541616-5x1Kit 5x1Kit
EUR 2675

CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504 1
EUR 270
Description: DNAse I from bovine pancreas (cow; Bos taurus)

CELLDATA RNAstorm™ Fresh Cell and Tissue RNA Isolation Kit, 50 preps

CD504-1 KT
EUR 270

ExoPure? Isolation Kit   (Urine)

K1240-10 each
EUR 836.4

ExoPure? Isolation Kit   (Urine)

K1240-2 each
EUR 483.6

Lipid Droplet Isolation Kit

MET-5011 50 preps
EUR 345

Lipid Droplet Isolation Kit

MBS169251-50Preps 50Preps
EUR 505

Lipid Droplet Isolation Kit

MBS169251-5x50Preps 5x50Preps
EUR 2320

Hyperspeed Total RNA Rapid Isolation Kit (whole blood, liquid samples)

MBS356004-50Tests 50Tests
EUR 335

Apoptotic Cell Isolation Kit

55R-1349 30 units
EUR 513
Description: Apoptotic Cell Isolation Kit for use in the research laboratory

miRNA ISOLATION KIT-TISSUE

IB47370 4 PREP KIT
EUR 30.42

miRNA ISOLATION KIT-TISSUE

IB47371 100 PREP KIT
EUR 579.66

Apoptotic Cell Isolation Kit

GWB-AXR172 30isolations Ask for price

Apoptotic Cell Isolation Kit

K258-30 each
EUR 561.6

EASYspin RNA Isolation Kit (For the whole blood)(Without Phenol & Chloroform)

MBS355949-50Tests 50Tests
EUR 435

EASYspin RNA Isolation Kit (For the whole blood)(Without Phenol & Chloroform)

MBS355949-5x50Tests 5x50Tests
EUR 1935

EASYspin RNA Isolation Kit (For bone tissues)(need not DNase I digestion)

MBS355955-50Tests 50Tests
EUR 495

EASYspin RNA Isolation Kit (For bone tissues)(need not DNase I digestion)

MBS355955-5x50Tests 5x50Tests
EUR 2235
Comparable developments have been noticed within the humic and nonhumic substances, the place several types of soil extract remedies influenced the concentrations of the fractions. Totally different soil extraction strategies can present additional particulars, thus widening the applying of soil extracts, particularly in microbes.

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